Nawata M, Minobe S, Watanabe T
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Yakugaku Zasshi. 1994 Oct;114(10):789-93. doi: 10.1248/yakushi1947.114.10_789.
The amount of endotoxin in the serum was measured by the new assay method of endotoxin using a filtercup, Limulus amebocyte lysate, and immobilized histidine which is a specific adsorbent for endotoxin. The maximum recovery of endotoxin in the rabbit serum was obtained using acetate buffer (pH 5.5, mu = 0.1) for the adsorption. Using this buffer, various kinds of endotoxin in water were adsorbed quantitatively on immobilized histidine, and the activity of the adsorbed endotoxin was well recovered. The value of the amount of endotoxin in the bovine serum measured by the new assay method, which includes heat treatment (70 degrees C, 10 min) after separation followed by washing, was closer to that calculated from pyrogenic activities in rabbits than that measured by the PCA-Toxicolor method.
采用一种新的内毒素检测方法来测定血清中的内毒素含量，该方法使用滤杯、鲎试剂和固定化组氨酸，其中固定化组氨酸是内毒素的特异性吸附剂。使用醋酸盐缓冲液（pH 5.5，μ = 0.1）进行吸附时，兔血清中内毒素的回收率最高。使用该缓冲液，水中的各种内毒素可被定量吸附到固定化组氨酸上，且吸附的内毒素活性能够很好地恢复。通过新检测方法测定的牛血清内毒素含量值，在分离后进行热处理（70℃，10分钟）并洗涤，该值比PCA - Toxicolor法测定的值更接近根据兔热源活性计算得出的值。
