Meyer C H, Bond J F, Chen M S, Kasaian M T
ImmuLogic Pharmaceutical Corporation, Waltham, MA 02154.
Clin Exp Allergy. 1994 Nov;24(11):1041-8. doi: 10.1111/j.1365-2222.1994.tb02741.x.
Allergy to the house dust mite Dermatophagoides pteronyssinus is mediated by IgE to the major allergens Der p I and Der p II in the majority of mite-allergic patients. In recent years, standardized preparations of D. pteronyssinus, commercially available from several sources, have become widely used for the diagnosis and immunotherapy of mite allergy. As standardization implies uniformity of allergen composition and potency, we directly compared the absolute and relative quantities of Der p I and Der p II in six different commercial standardized extracts of D. pteronyssinus. Our findings reveal variability in levels of both Der p I and Der p II, producing ratios of Der p I/Der p II ranging from 1.1/1 to 6/1. Although the content of minor allergens in the extracts was not evaluated here, their contribution to the overall reactivity of mite-allergic patients to the commercial extracts was judged to be minimal. This was demonstrated by showing that plasma depleted of reactivity to both Der p I and Der p II had virtually no residual IgE directed against extract components. The variation in the proportion of Der p I and Der p II among different D. pteronyssinus extracts is likely to influence their biological effectiveness. Patients with reactivity against only Der p I or Der p II, who were found to comprise approximately one-third of the mite-allergic population, may not respond optimally to extracts containing relatively low levels of the allergen to which they are sensitive.
在大多数对螨过敏的患者中，对屋尘螨（粉尘螨）的过敏反应是由针对主要变应原Der p I和Der p II的IgE介导的。近年来，有多个来源可商购的标准化粉尘螨制剂已广泛用于螨过敏的诊断和免疫治疗。由于标准化意味着变应原组成和效力的一致性，我们直接比较了六种不同的市售标准化粉尘螨提取物中Der p I和Der p II的绝对量和相对量。我们的研究结果显示，Der p I和Der p II的水平存在差异，Der p I/Der p II的比值范围为1.1/1至6/1。尽管此处未评估提取物中次要变应原的含量，但据判断它们对螨过敏患者对市售提取物的总体反应性的贡献极小。这一点通过以下方式得到证明：去除了对Der p I和Der p II反应性的血浆实际上没有针对提取物成分的残留IgE。不同粉尘螨提取物中Der p I和Der p II比例的变化可能会影响它们的生物学效力。仅对Der p I或Der p II有反应性的患者（发现约占螨过敏人群的三分之一）可能对含有相对低水平其敏感变应原的提取物反应不佳。
