Xie Q, Jiménez A
Centro de Biología Molecular Severo Ochoa (U.A.M./C.S.I.C.), Universidad Autónoma, Cantoblanco, Madrid, Spain.
Curr Genet. 1994 Nov-Dec;26(5-6):398-402. doi: 10.1007/BF00309925.
Two different ILV5 genes encoding acetohydroxy-acid isomeroreductases, and named ILV5G and ILV5X, were cloned and sequenced from a Saccharomyces cerevisiae brewing strain. The coding sequence of ILV5X shows a single nucleotide change with respect to that from the ILV5 gene of a S. cerevisiae laboratory strain. In addition, all promoter motifs which are, or are presumed to be, implicated in transcription regulatory functions are identical in ILV5 and ILV5X. In contrast, the coding sequence of ILV5G differs in 5.6% of its nucleotides from that of ILV5 and most of its promoter regulatory motifs show a single nucleotide change with respect to those from ILV5.
从一株酿酒酵母菌株中克隆并测序了两个不同的编码乙酰羟酸异构还原酶的ILV5基因，分别命名为ILV5G和ILV5X。与酿酒酵母实验室菌株的ILV5基因相比，ILV5X的编码序列有一个单核苷酸变化。此外，所有参与转录调控功能的或被推测参与转录调控功能的启动子基序在ILV5和ILV5X中是相同的。相比之下，ILV5G的编码序列有5.6%的核苷酸与ILV5不同，并且其大多数启动子调控基序与ILV5相比都有一个单核苷酸变化。
