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Characterization of the ilv-2 gene from Neurospora crassa encoding alpha-keto-beta-hydroxylacyl reductoisomerase.

作者信息

Sista H, Bowman B

机构信息

Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064.

出版信息

Gene. 1992 Oct 12;120(1):115-8. doi: 10.1016/0378-1119(92)90018-k.

DOI:10.1016/0378-1119(92)90018-k
PMID:1398116
Abstract

We have isolated the cDNA and corresponding genomic DNA for the ilv-2 locus of Neurospora crassa. This gene encodes alpha-keto-beta-hydroxylacyl reductoisomerase (Ilv-2), required for the synthesis of isoleucine and valine. The gene contains four introns, maps to the right arm of chromosome V, and encodes a protein of 400 amino acids (aa). Alignment of the aa sequence of Ilv-2 with the two other known eukaryotic sequences encoding this enzyme reveals two conserved regions.

摘要

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Characterization of the ilv-2 gene from Neurospora crassa encoding alpha-keto-beta-hydroxylacyl reductoisomerase.
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