粟酒裂殖酵母组蛋白基因的物理图谱

Physical mapping of the Schizosaccharomyces pombe histone genes.

作者信息

Lind M, Lunderius C, Ekwall K, Olsson T

机构信息

Department of Molecular Biology, Biomedicum, Uppsala, Sweden.

出版信息

Curr Genet. 1994 Nov-Dec;26(5-6):553-6. doi: 10.1007/BF00309949.

DOI:10.1007/BF00309949

PMID:7874752

Abstract

The histone-encoding genes in Schizosaccharomyces pombe were physically mapped by hybridisation to filters containing cosmid and P1 genomic libraries. The H2A.2 gene and the H2A.1-H2B.1 gene pair mapped between the ade6 and rikI genes on chromosome III. The three H4-H3 gene pairs were mapped to three different regions by a H4.1 probe. Southern analysis of clones from each region revealed the positions of the three H4-H3 gene pairs. H4.1-H3.1 was localised to chromosome I between the mei2 and rad1 genes; H4.2-H3.2 mapped between rad3 and cdc2 on chromosome II; H4.3-H3.3 was localised to a region between the nuc1 and puc1 genes on chromosome II.

摘要

通过与包含黏粒和P1基因组文库的滤膜杂交，对粟酒裂殖酵母中的组蛋白编码基因进行了物理定位。H2A.2基因和H2A.1 - H2B.1基因对定位于第三条染色体上的ade6和rikI基因之间。通过H4.1探针将三对H4 - H3基因对定位于三个不同区域。对每个区域的克隆进行Southern分析，揭示了三对H4 - H3基因对的位置。H4.1 - H3.1定位于第一条染色体上的mei2和rad1基因之间；H4.2 - H3.2定位于第二条染色体上的rad3和cdc2之间；H4.3 - H3.3定位于第二条染色体上的nuc1和puc1基因之间的一个区域。