Bernardi F, Koller T, Thoma F
Institut für Zellbiologie, ETH-Hönggerberg, Zürich, Switzerland.
Yeast. 1991 Aug-Sep;7(6):547-58. doi: 10.1002/yea.320070603.
We have analysed the chromatin structure of the ade6 gene of Schizosaccharomyces pombe and its flanking regions both in the chromosome and in plasmids. The chromatin structure is independent of the chromosomal or extrachromosomal location. The ade6 gene contains eight precisely positioned nucleosomes on the 5' half, 'not positioned' nucleosomes around the 3' end and a nuclease-sensitive promoter region. Precisely positioned nucleosomes, but no nuclease-sensitive region were also detected on the ura4 gene in the chromosome and on a plasmid. The results show that S. pombe chromosomal and extrachromosomal genes have chromatin structures similar to those of S. cerevisiae and higher eukaryotes.
我们分析了裂殖酵母ade6基因及其侧翼区域在染色体和质粒中的染色质结构。染色质结构与染色体或染色体外的位置无关。ade6基因在5'端一半含有八个精确定位的核小体，3'端周围有“未定位”的核小体以及一个核酸酶敏感的启动子区域。在染色体和质粒中的ura4基因上也检测到了精确定位的核小体，但没有核酸酶敏感区域。结果表明，裂殖酵母的染色体和染色体外基因具有与酿酒酵母和高等真核生物相似的染色质结构。
