Harrell L J, Mirrett S, Reller L B
Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina, 27710.
Diagn Microbiol Infect Dis. 1994 Nov;20(3):121-5. doi: 10.1016/0732-8893(94)90105-8.
A prospective study was done to assess the comparative use of acridine orange and Gram stains for detecting false-positive BACTEC blood culture bottles, thereby eliminating unnecessary subcultures. A total of 1049 NR 6A and 7A bottles that were instrument-positive in the BACTEC 660 nonradiometric system, but were Gram stain-negative, had aerobic and anaerobic subcultures as well as an acridine orange stain performed. Only five of 1049 (0.5%) instrument-positive, Gram stain-negative bottles grew microorganisms on subculture. Of these five microorganisms, acridine orange stain detected two. All five microorganisms were assessed not to be clinically significant based on review of the patients' charts. Our data showed that the Gram stain and the acridine orange stain are comparable for detecting false-positive NR 6A and 7A bottles. We conclude that subculture of BACTEC-positive, Gram stain-negative NR 6A and 7A bottles is not necessary.
开展了一项前瞻性研究，以评估吖啶橙染色和革兰氏染色在检测BACTEC血培养瓶假阳性方面的比较应用，从而消除不必要的传代培养。在BACTEC 660非放射性系统中仪器检测呈阳性但革兰氏染色呈阴性的1049个NR 6A和7A瓶，进行了需氧和厌氧传代培养以及吖啶橙染色。1049个仪器检测呈阳性、革兰氏染色呈阴性的瓶中，只有5个（0.5%）在传代培养时培养出微生物。在这5种微生物中，吖啶橙染色检测出2种。根据对患者病历的审查，所有5种微生物均被评估为无临床意义。我们的数据表明，革兰氏染色和吖啶橙染色在检测NR 6A和7A瓶假阳性方面具有可比性。我们得出结论，BACTEC检测呈阳性、革兰氏染色呈阴性的NR 6A和7A瓶无需进行传代培养。
