Boucherié H, Bataille N, Fitch I T, Perrot M, Tuite M F
Laboratoire de Génétique, UA CNRS 542, Université de Bordeaux II, Talence, France.
FEMS Microbiol Lett. 1995 Jan 15;125(2-3):127-33. doi: 10.1111/j.1574-6968.1995.tb07348.x.
Three unlinked genes, TDH1, TDH2 and TDH3, encode the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (triose-phosphate dehydrogenase; TDH) in the yeast Saccharomyces cerevisiae. We demonstrate that the synthesis of the three encoded TDH polypeptides (TDHa, TDHb and TDHc, respectively) is not co-ordinately regulated and that TDHa is only synthesised as cells enter stationary phase, due to glucose starvation, or in heat-shocked cells. Furthermore, the synthesis of TDHb, but not TDHc, is strongly repressed by a heat shock. Hence, the TDHa enzyme may play a cellular role, distinct from glycolysis, that is required by stressed cells.
三个不连锁的基因TDH1、TDH2和TDH3，在酿酒酵母中编码糖酵解酶甘油醛-3-磷酸脱氢酶（磷酸丙糖脱氢酶；TDH）。我们证明，三种编码的TDH多肽（分别为TDHa、TDHb和TDHc）的合成并非协同调节，并且由于葡萄糖饥饿，TDHa仅在细胞进入稳定期时合成，或者在热激细胞中合成。此外，热激强烈抑制TDHb的合成，但不抑制TDHc的合成。因此，TDHa酶可能发挥着与糖酵解不同的细胞作用，这是应激细胞所需要的。
