McAlister L, Holland M J
J Biol Chem. 1985 Dec 5;260(28):15013-8.
Mutant yeast strains were constructed which carry insertion mutations in each of the glyceraldehyde-3-phosphate dehydrogenase structural genes which have been designated TDH1, TDH2, and TDH3. Haploid strains carrying mutations in TDH1 and TDH2 as well as TDH1 and TDH3 were isolated from crosses between strains carrying the appropriate single mutations. The three single mutants as well as the two double mutants grow at wild type rates when ethanol is used as carbon source. Mutant strains lacking only a functional TDH2 allele or a TDH3 allele grow at 50 and 75% of the rate observed for wild type cells, respectively, when glucose is used as carbon source. No growth phenotype was observed for strains lacking only a functional TDH1 allele when either fermentable or nonfermentable carbon sources were used. Evidence is presented that strains lacking functional TDH2 and TDH3 alleles are not viable. These data demonstrate that the presence of a functional TDH2 or TDH3 allele is required for cell growth.
构建了突变酵母菌株，这些菌株在已命名为TDH1、TDH2和TDH3的每个甘油醛-3-磷酸脱氢酶结构基因中携带插入突变。从携带适当单突变的菌株之间的杂交中分离出在TDH1和TDH2以及TDH1和TDH3中携带突变的单倍体菌株。当乙醇用作碳源时，三个单突变体以及两个双突变体以野生型速率生长。当葡萄糖用作碳源时，仅缺乏功能性TDH2等位基因或TDH3等位基因的突变菌株分别以野生型细胞观察到的速率的50%和75%生长。当使用可发酵或不可发酵碳源时，仅缺乏功能性TDH1等位基因的菌株未观察到生长表型。有证据表明，缺乏功能性TDH2和TDH3等位基因的菌株无法存活。这些数据表明，细胞生长需要功能性TDH2或TDH3等位基因的存在。
