Sellstedt A
Department of Plant Physiology, University of Umeå, Sweden.
FEMS Microbiol Lett. 1995 Jan 15;125(2-3):231-6. doi: 10.1111/j.1574-6968.1995.tb07363.x.
Nodulation ability was tested for Frankia strains HFPCcI3 and EL1, and Frankia sources A.t. and G.a. from Allocasaurina torulosa and Gymnostoma australianum, respectively, on A. torulosa Miq., Casuarina cunninghamiana Miq., G. australianum L. Johnson and Elaeagnus triflora Roxb. It was shown that A. torulosa and C. cunninghamiana formed nodules only with the Frankia sources obtained from their own host plant, while E. triflora formed nodules with three of the four Frankia sources tested. All nodules formed were effectively fixing nitrogen. Specific nitrogenase activity was highest in E. triflora inoculated with the Frankia strain isolated from nodules of the same species. Identification of Frankia sources in the nodules was performed by use of PCR amplification of DNA with a random primer. PCR amplification of DNA isolated from nodules of G. australianum and E. triflora inoculated with Frankia strain EL1 revealed, when compared with DNA amplified from free living Frankia strain EL1, that there was only one Frankia strain causing the observed nodules.
对弗氏放线菌菌株HFPCcI3和EL1，以及分别来自圆叶Allocasaurina torulosa和澳洲裸孔桉Gymnostoma australianum的弗氏放线菌来源A.t.和G.a.，在圆叶Allocasaurina torulosa Miq.、细叶木麻黄Casuarina cunninghamiana Miq.、澳洲裸孔桉Gymnostoma australianum L. Johnson和胡颓子Elaeagnus triflora Roxb.上进行了结瘤能力测试。结果表明，圆叶Allocasaurina torulosa和细叶木麻黄仅与从其自身寄主植物获得的弗氏放线菌来源形成根瘤，而胡颓子与所测试的四种弗氏放线菌来源中的三种形成根瘤。所有形成的根瘤都能有效固氮。接种从同一物种根瘤中分离出的弗氏放线菌菌株的胡颓子中，固氮酶的比活性最高。通过使用随机引物对DNA进行PCR扩增，对根瘤中的弗氏放线菌来源进行了鉴定。与从自由生活的弗氏放线菌菌株EL1扩增的DNA相比，对接种弗氏放线菌菌株EL1的澳洲裸孔桉和胡颓子根瘤中分离的DNA进行PCR扩增显示，只有一种弗氏放线菌菌株导致观察到的根瘤形成。
