Simonet P, Normand P, Moiroud A, Bardin R
Laboratoire de Biologie des Sols, U.A. CNRS 697, Université Lyon 1, Villeurbanne, France.
Arch Microbiol. 1990;153(3):235-40. doi: 10.1007/BF00249074.
A set of oligonucleotides has been developed to study the competitivity of two Frankia strains in the nodulation of the roots of two host plant species: Alnus glutinosa and Alnus incana. Two 20 mer-oligonucleotides, complementary to highly conserved sequences inside the nifH gene, were used as primers for the polymerase chain reaction (PCR) system in order to amplify microsymbiont DNA extracted from actinorhizae. PCR products were analyzed using two strain-specific 15-mer oligonucleotides identified in the amplified region. Hybridization data indicate that strain ACoN24d is more competitive than strain ArI3 in the nodulation of both hosts.
已开发出一组寡核苷酸，用于研究两种弗兰克氏菌菌株在两种宿主植物物种（欧洲桤木和灰桤木）根瘤形成中的竞争力。两种与nifH基因内部高度保守序列互补的20聚体寡核苷酸被用作聚合酶链反应（PCR）系统的引物，以扩增从放线根瘤中提取的微共生体DNA。使用在扩增区域鉴定出的两种菌株特异性15聚体寡核苷酸对PCR产物进行分析。杂交数据表明，在两种宿主的根瘤形成中，菌株ACoN24d比菌株ArI3更具竞争力。
