Palacios R
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6652-6. doi: 10.1073/pnas.82.19.6652.
The monoclonal antibodies (mAb) DA6.147, DA6.164, and HIG.48 against human Ia antigens, but not the W6/32 mAb against human class I major histocompatibility complex antigens or the anti-monocyte OKM1 and 63D3 mAb, stimulated monocytes to secrete interleukin 1 (IL-1). IL-1 was measured by its property of promoting the production of interleukin 2 (IL-2) by phytohemagglutinin-treated LBRM-33 clone 1A5 cells. IL-1 activity induced by anti-Ia antibodies could be detected 24 hr after initiation of the cultures and reached its highest levels at days 3-4 of culture. Concentrations of 1 microgram/ml or higher of the anti-Ia antibodies induced monocytes to secrete significant levels of IL-1 activity. The anti-Ia mAb induced Ia-bearing but not Ia-negative monocytes to secrete IL-1. Both Ia-positive and Ia-negative monocytes produced IL-1 activity under the stimulus of lipopolysaccharide. It is concluded that the DA6.147, DA6.164, and HIG.48 mAb stimulate secretion of IL-1 by interacting Ia antigens on monocytes. The data support the view that besides serving as restricting elements for recognition of foreign antigens by T cells, Ia antigens may also function as transducer elements.
抗人Ia抗原的单克隆抗体（mAb）DA6.147、DA6.164和HIG.48可刺激单核细胞分泌白细胞介素1（IL-1），而抗人I类主要组织相容性复合体抗原的W6/32 mAb以及抗单核细胞OKM1和63D3 mAb则不能。通过检测其促进经植物血凝素处理的LBRM-33克隆1A5细胞产生白细胞介素2（IL-2）的特性来测定IL-1。抗Ia抗体诱导的IL-1活性在培养开始后24小时即可检测到，并在培养的第3至4天达到最高水平。浓度为1微克/毫升或更高的抗Ia抗体可诱导单核细胞分泌显著水平的IL-1活性。抗Ia mAb诱导表达Ia的单核细胞而非不表达Ia的单核细胞分泌IL-1。在脂多糖刺激下，Ia阳性和Ia阴性单核细胞均可产生IL-1活性。得出结论，DA6.147、DA6.164和HIG.48 mAb通过与单核细胞上的Ia抗原相互作用刺激IL-1的分泌。这些数据支持这样一种观点，即Ia抗原除了作为T细胞识别外来抗原的限制元件外，还可能作为转导元件发挥作用。
