Barker F G, Ozua P, Van Noorden S
Department of Histopathology, Royal Postgraduate Medical School, London.
J Clin Pathol. 1994 Dec;47(12):1118-9. doi: 10.1136/jcp.47.12.1118.
False positive immunostaining for cytomegalovirus in products of conception was revealed using an avidinbiotinylated peroxidase complex. The cause was shown to be endogenous biotin. The use of a non-avidin-biotin method avoided the problem.
采用抗生物素蛋白-生物素化过氧化物酶复合物法检测妊娠产物中的巨细胞病毒时出现了假阳性免疫染色。结果表明原因是内源性生物素。使用非抗生物素蛋白-生物素方法可避免该问题。