Yokoyama S, Kashima K, Inoue S, Daa T, Nakayama I, Moriuchi A
Department of Laboratory Medicine, Medical College of Oita, Japan.
Am J Clin Pathol. 1993 Jan;99(1):13-7. doi: 10.1093/ajcp/99.1.13.
Intranuclear inclusions sometimes are observed in endometrial glands, especially during puerperium, and superficially they resemble those from herpesvirus-infected cells. To study the significance of the inclusions, endometrial and placental tissue from 215 subjects, including 69 pregnancy-associated samples, were examined. With screening by peroxidase-labeled avidin alone, followed by the peroxidase reaction, only pregnancy-related endometrium (in 32 cases) demonstrated reactivity in the intranuclear inclusions. These intranuclear inclusions demonstrated positive immunostaining for biotin using the peroxidase-antiperoxidase method in all but six cases, whereas there was no reaction for Herpes simplex in any of the cases. The endometrial tissues of the 32 cases were obtained from the 16th gestational week to the 37th postpartum day. Although the histogenesis of these intranuclear inclusions has not been clarified, they should not be mistaken for those in endometrial cells infected by herpesvirus because of intranuclear biotin and a false-positive histochemical reaction in the avidin-biotin-peroxidase complex method.
核内包涵体有时可见于子宫内膜腺体，尤其是在产褥期，表面上它们类似于疱疹病毒感染细胞中的包涵体。为研究这些包涵体的意义，对215名受试者的子宫内膜和胎盘组织进行了检查，其中包括69份与妊娠相关的样本。仅用过氧化物酶标记的抗生物素蛋白进行筛查，随后进行过氧化物酶反应，仅妊娠相关的子宫内膜（32例）在核内包涵体中显示出反应性。除6例病例外，这些核内包涵体在所有病例中使用过氧化物酶-抗过氧化物酶方法对生物素均显示阳性免疫染色，而所有病例中单纯疱疹均无反应。这32例病例的子宫内膜组织取自妊娠第16周直至产后第37天。尽管这些核内包涵体的组织发生尚未阐明，但由于核内生物素以及抗生物素蛋白-生物素-过氧化物酶复合物方法中的假阳性组织化学反应，不应将它们误认为是疱疹病毒感染的子宫内膜细胞中的包涵体。
