Sickel J Z, di Sant'Agnese P A
Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, NY.
Arch Pathol Lab Med. 1994 Aug;118(8):831-3.
A distinctive morphologic alteration originally described as nuclear "clearing" has been observed in endometrial epithelium during pregnancy. Affected nuclei have a clear center and a rim of marginated chromatin, imparting a "glassy" appearance reminiscent of herpesvirus inclusions. We observed this change in gestational endometrium attached to the membranes of an otherwise normal-term placenta. Because of the morphologic suggestion of herpesvirus infection, immunoperoxidase studies using the avidin-biotin method were performed, resulting in intense staining of nuclear inclusions. An identical pattern of nuclear staining was also noted in negative controls. This anomalous staining reaction was replicated with the streptavidin-biotin method but not with the peroxidase-antiperoxidase method. Furthermore, nuclear staining was abolished after sequential preincubation with free avidin (0.05%) and free biotin (0.05%). Using the peroxidase-antiperoxidase method, positive nuclear staining was identified with monoclonal antibodies to biotin. Similar results were obtained in nine additional cases of optically clear nuclei associated with term placentas. We conclude that staining of optically clear nuclei is the result of avidin and streptavidin binding to intranuclear biotin. This anomalous staining pattern could potentially result in the misdiagnosis of herpesvirus infection.
在孕期子宫内膜上皮中观察到一种独特的形态学改变，最初被描述为核“空泡化”。受影响的细胞核有一个清晰的中心和一圈边缘化的染色质，呈现出一种“玻璃样”外观，让人联想到疱疹病毒包涵体。我们在与正常足月胎盘胎膜相连的妊娠子宫内膜中观察到了这种变化。由于形态学上提示疱疹病毒感染，我们采用抗生物素蛋白-生物素方法进行了免疫过氧化物酶研究，结果核包涵体出现强烈染色。在阴性对照中也观察到了相同的核染色模式。这种异常染色反应在用链霉抗生物素蛋白-生物素方法时可重复出现，但用过氧化物酶-抗过氧化物酶方法时则不会出现。此外，在用游离抗生物素蛋白（0.05%）和游离生物素（0.05%）依次预孵育后，核染色消失。用过氧化物酶-抗过氧化物酶方法，用抗生物素蛋白单克隆抗体可识别出阳性核染色。在另外9例与足月胎盘相关的光学透明细胞核病例中也得到了类似结果。我们得出结论，光学透明细胞核的染色是抗生物素蛋白和链霉抗生物素蛋白与核内生物素结合的结果。这种异常染色模式可能会导致疱疹病毒感染的误诊。
