Ali R A, Qureshi M A, McCorkle F M
Department of Poultry Science, North Carolina State University, Raleigh 27695-7608.
Immunopharmacol Immunotoxicol. 1994 Nov;16(4):611-25. doi: 10.3109/08923979409019742.
The effects of catecholamines (CA) on various chicken macrophage functions were examined. Macrophage monolayers were exposed to .01, .1, .25, 1, 2, and 5 micrograms/mL of dopamine (DA), norepinephrine (NE) and epinephrine (E) for 1 hr. All CA were toxic for macrophages at 1-5 micrograms dose range resulting in 25-50% cell death. All CA at the .1 and .25 micrograms/mL level increased E. coli and sheep red blood cells (SRBC) phagocytosis by macrophages. The percentage of Fc-receptor positive macrophages increased after CA exposure. Prolonged exposure of macrophages (3 hr) reduced SRBC phagocytosis by DA-treated but not in NE- and E-treated macrophages. However, after 1 hr exposure and 3 hr recovery period, CA-induced changes were reversed in all but DA-treated cultures. Apomorphine and metoclopromide blocked DA whereas propranolol blocked NE and E effects suggesting specificity of the observed effects via catecholaminergic receptors on chicken macrophages. Dopamine and NE (.25 micrograms/mL) did not affect but E exposure enhanced LPS-induced tumoricidal factor production. These findings suggest that CA modulate chicken macrophage effector functions.
研究了儿茶酚胺（CA）对鸡巨噬细胞各种功能的影响。将巨噬细胞单层暴露于0.01、0.1、0.25、1、2和5微克/毫升的多巴胺（DA）、去甲肾上腺素（NE）和肾上腺素（E）中1小时。在1 - 5微克剂量范围内，所有CA对巨噬细胞均有毒性，导致25 - 50%的细胞死亡。在0.1和0.25微克/毫升水平时，所有CA均增加巨噬细胞对大肠杆菌和绵羊红细胞（SRBC）的吞噬作用。CA暴露后，Fc受体阳性巨噬细胞的百分比增加。巨噬细胞长时间暴露（3小时）会降低DA处理组对SRBC的吞噬作用，但NE和E处理组则不会。然而，在暴露1小时和恢复期3小时后，除DA处理的培养物外，所有CA诱导的变化均会逆转。阿扑吗啡和甲氧氯普胺可阻断DA的作用，而普萘洛尔可阻断NE和E的作用，这表明通过儿茶酚胺能受体对鸡巨噬细胞产生的观察到的效应具有特异性。多巴胺和NE（0.25微克/毫升）不影响，但E暴露会增强脂多糖诱导的肿瘤杀伤因子的产生。这些发现表明CA可调节鸡巨噬细胞的效应功能。
