Muramatsu H
Department of Clinical Pathology, Kitasato University School of Medicine.
Kansenshogaku Zasshi. 1994 Dec;68(12):1465-71. doi: 10.11150/kansenshogakuzasshi1970.68.1465.
A nested PCR assay was developed for the diagnosis of Candida albicans infection. A segment of the C. albicans beta-tubulin gene was amplified in this assay that was sensitive with the detection limit of 10 fg DNA of C. albicans. This assay was found to be specific by obtaining the negative results with 13 organisms (5 other Candida, 2 Cryptococcus, 3 Aspergillus, Escherichia coli and others). Cerebrospinal fluid of a patient with C. albicans meningitis was analyzed and was found to be positive. These results suggest that this assay can be clinically useful because of its high sensitivity, specificity and rapidity.
开发了一种巢式聚合酶链反应（PCR）检测法用于诊断白色念珠菌感染。在此检测法中扩增了白色念珠菌β-微管蛋白基因的一个片段，该检测法灵敏度高，白色念珠菌DNA的检测限为10 fg。通过对13种微生物（5种其他念珠菌、2种隐球菌、3种曲霉菌、大肠杆菌等）检测获得阴性结果，证明该检测法具有特异性。对一名患有白色念珠菌脑膜炎患者的脑脊液进行分析，结果呈阳性。这些结果表明，该检测法因其高灵敏度、特异性和快速性而具有临床应用价值。
