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通过聚合酶链反应检测人血液中白色念珠菌的新方法。

New method for detection of Candida albicans in human blood by polymerase chain reaction.

作者信息

Miyakawa Y, Mabuchi T, Fukazawa Y

机构信息

Department of Microbiology, Yamanashi Medical College, Japan.

出版信息

J Clin Microbiol. 1993 Dec;31(12):3344-7. doi: 10.1128/jcm.31.12.3344-3347.1993.

DOI:10.1128/jcm.31.12.3344-3347.1993
PMID:8308135
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266430/
Abstract

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.

摘要

我们开发了一种通过聚合酶链反应检测人血液中白色念珠菌细胞的新方法，该反应可扩增物种特异性多拷贝DNA片段内的一个125碱基对区域。在从含有白色念珠菌的血液中制备模板DNA时，加入热带念珠菌细胞以及与两种细胞都发生反应的抗体，可将白色念珠菌的检测灵敏度提高到每0.1毫升约3个细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb56/266430/1f3432527b23/jcm00024-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb56/266430/bd0392f8cb5f/jcm00024-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb56/266430/1f3432527b23/jcm00024-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb56/266430/bd0392f8cb5f/jcm00024-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb56/266430/1f3432527b23/jcm00024-0282-a.jpg

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New method for detection of Candida albicans in human blood by polymerase chain reaction.通过聚合酶链反应检测人血液中白色念珠菌的新方法。
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本文引用的文献

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Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans.抗白色念珠菌主要抗原因子的凝集性单克隆抗体的制备与鉴定
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Rapid identification of yeasts in positive blood cultures by a multiplex PCR method.通过多重聚合酶链反应方法快速鉴定阳性血培养中的酵母菌。
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Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis.通过聚合酶链反应（PCR）简单快速地检测血清中白色念珠菌DNA以诊断侵袭性念珠菌病。
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Rapid detection and identification of Candida, Aspergillus, and Fusarium species in ocular samples using nested PCR.使用巢式聚合酶链反应快速检测和鉴定眼部样本中的念珠菌、曲霉菌和镰刀菌属菌种。
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The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis.聚合酶链反应。一种利用分子遗传学进行医学诊断的新方法。
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