Zierdt C H, Zierdt W S, Nagy B
Clinical Pathology Department, National Institutes of Health, Bethesda, Maryland 20892.
J Parasitol. 1995 Feb;81(1):127-9.
An enzyme-linked immunosorbent assay was devised in order to search for antibodies against Blastocystis hominis in infected humans. Reaction proteins were obtained from washed, axenic B. hominis cells, as sonicate. Sonicate was diluted to provide 17 and 34 micrograms of protein per well. Dilutions of patients' sera were applied, followed by phosphatase-conjugated goat anti-human serum and phosphatase substrate. Color was measured at 405 microns wavelength. Immunoglobulin G antibodies to high titers were found. Of 30 sera tested from 28 patients, 3 were negative at the 1/50 threshold dilution, 8 were positive at 1/50, 3 at 1/100, 2 at 1/200, 3 at 1/400, 6 at 1/800, and 5 at 1/1,600. Normal sera (42 blood bank sera) were all negative at 1/50. Each serum was subjected to multiple testing. Duplicate tests were included for each run, and runs were made from 4 to 6 for each serum. Blastocystis hominis is increasingly recognized to be a cause of human enteric disease, with symptoms often like those in giardiasis. Demonstration of strong antibody response is consistent with this view.
为了在受感染的人类中寻找抗人芽囊原虫的抗体，设计了一种酶联免疫吸附测定法。反应蛋白取自经洗涤的无菌人芽囊原虫细胞的超声裂解物。将超声裂解物稀释，使每孔含有17微克和34微克蛋白质。加入患者血清的稀释液，随后加入磷酸酶偶联的山羊抗人血清和磷酸酶底物。在405微米波长处测量吸光度。发现了高滴度的免疫球蛋白G抗体。在检测的来自28名患者的30份血清中，3份在1/50的临界稀释度下为阴性，8份在1/50时为阳性，3份在1/100时为阳性，2份在1/200时为阳性，3份在1/400时为阳性，6份在1/800时为阳性，5份在1/1600时为阳性。正常血清（42份血库血清）在1/50时均为阴性。每份血清都进行了多次检测。每次检测都包括重复试验，每份血清进行4至6次检测。人芽囊原虫越来越被认为是人类肠道疾病的病因，其症状通常与贾第虫病相似。强烈抗体反应的证明与这一观点一致。
