Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., Abbassia, 11566, Cairo, Egypt.
Parasitol Res. 2011 May;108(5):1139-46. doi: 10.1007/s00436-010-2156-8. Epub 2010 Dec 7.
The present study was performed to characterize the protein profiles of Blastocystis hominis isolates from symptomatic and asymptomatic individuals by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from symptomatic and asymptomatic patients. The presence of immunogenic bands associated with pathogenicity or of diagnostic potentials was also evaluated. The study comprised 80 individuals classified into four groups, 20 each: symptomatic blastocystosis (G1), asymptomatic blastocystosis (G2), other parasitic infections (G3), and healthy control subjects (G4). SDS-PAGE analysis of individual antigens form symptomatic and asymptomatic B. hominis isolates revealed similar and distinctive antigenic bands with significant differences in two high (123.5 and 112.3 kDa) and few low molecular weight bands (48.5, 38, 42.3, and 35.5 kDa). Immunoblotting was performed using symptomatic and asymptomatic antigen pools with sera of the four studied groups. It was found that anti-B. hominis IgG reacted with nine protein bands ranging from 100 to 18 kDa of the symptomatic antigen pool. There was a significant difference between G1 and G2 in the recognition of 64, 56, 38, and 29 kDa antigen bands. Also, anti-B. hominis IgG reacted with five protein bands ranging from 56 to 12 kDa of asymptomatic antigen pool. There was a significant difference between G1 and G2 in the recognition of 29 kDa antigen band. These findings suggest the potential use of the 29-kDa antigen as marker of pathogenicity and implicate its use in the diagnosis and differentiation between symptomatic and asymptomatic blastocystosis.
本研究通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹，使用来自有症状和无症状患者的血清，对来自有症状和无症状个体的溶组织内阿米巴蛋白图谱进行了特征分析。还评估了与致病性相关或具有诊断潜力的免疫原性条带的存在。该研究包括 80 名个体，分为 4 组，每组 20 名：有症状的溶组织内阿米巴病（G1）、无症状的溶组织内阿米巴病（G2）、其他寄生虫感染（G3）和健康对照组（G4）。对来自有症状和无症状的溶组织内阿米巴分离株的个体抗原进行 SDS-PAGE 分析，显示出相似和独特的抗原带，在两个高（123.5 和 112.3 kDa）和少数低分子量带（48.5、38、42.3 和 35.5 kDa）方面存在显著差异。用来自四个研究组的血清对有症状和无症状抗原池进行免疫印迹。发现抗溶组织内阿米巴 IgG 与来自有症状抗原池的 9 条蛋白带（分子量范围为 100 至 18 kDa）发生反应。在 G1 和 G2 之间，对 64、56、38 和 29 kDa 抗原带的识别存在显著差异。此外，抗溶组织内阿米巴 IgG 与无症状抗原池的 5 条蛋白带（分子量范围为 56 至 12 kDa）发生反应。在 G1 和 G2 之间，对 29 kDa 抗原带的识别存在显著差异。这些发现表明 29 kDa 抗原可作为致病性标志物，并暗示其可用于诊断和区分有症状和无症状的溶组织内阿米巴病。
