Wikesjö U M, Kean C J, Zimmerman G J
Advanced Education Program in Periodontics, Loma Linda University, School of Dentistry, CA.
J Periodontol. 1994 Dec;65(12):1151-7. doi: 10.1902/jop.1994.65.12.1151.
It is increasingly evident that controlled preclinical models with reproducible defect characteristics and biologic reaction are critical for evaluation of safety and efficacy of periodontal reconstructive protocols. Our investigations have characterized a supraalveolar periodontal defect in the mandibular premolar region in the beagle dog. This surgically-created critical size defect heals with almost complete connective tissue attachment following immediate reconstructive surgery. When the defect is exposed to periodontitis-simulating conditions prior to surgery the new connective tissue attachment is reduced. Bone and cementum regeneration is limited for both defect variations. Critical aspects relative to animal selection and management, surgical protocol, histologic and statistical analysis are discussed. This preclinical model has proven effective for evaluation of safety and efficacy of devices and biologics used adjunctive to periodontal reconstructive therapy.
越来越明显的是，具有可重复的缺损特征和生物学反应的可控临床前模型对于评估牙周重建方案的安全性和有效性至关重要。我们的研究已经对比格犬下颌前磨牙区域的牙槽嵴上方牙周缺损进行了特征描述。这种通过手术制造的临界尺寸缺损在即刻重建手术后几乎完全通过结缔组织附着愈合。当缺损在手术前暴露于模拟牙周炎的条件下时，新的结缔组织附着会减少。两种缺损变体的骨和牙骨质再生均有限。讨论了与动物选择和管理、手术方案、组织学和统计分析相关的关键方面。这种临床前模型已被证明对于评估牙周重建治疗辅助使用的器械和生物制品的安全性和有效性是有效的。
