Daniels W M, Jaffer A, Russell V A, Taljaard J J
Department of Chemical Pathology, University of Stellenbosch, Tygerberg Hospital.
Neurochem Res. 1994 Dec;19(12):1539-43. doi: 10.1007/BF00969003.
Bipolar electrodes were implanted into the CA1 pyramidal cells of the dorsal hippocampus and the effect of electrical stimulation of these cells on corticosterone secretion was investigated in freely moving rats. Histology showed that the electrodes were positioned in close proximity to the CA1 pyramidal cells. Rats that were subjected to high intensity electrical stimulation (1, 10, and 100 microA) behaved differently when compared to their sham stimulated controls. They were more active and displayed wet dog shakes. Plasma corticosterone levels increased dose-dependently in rats subjected to different electrical stimulation intensities. Although prior treatment (24 hours) of rats with DSP4 (60 mg/kg, i.p.) significantly reduced hippocampal noradrenaline content by 46%, it did not bring about any behavioural changes. DSP4 treatment also had no effect on electrically stimulated corticosterone release. These data suggested that stimulation of CA1 pyramidal cells may lead to increased corticosterone release and that a decrease in hippocampal noradrenaline concentration was unable to alter this corticosterone response.
将双极电极植入背侧海马体的CA1锥体细胞中，研究了对这些细胞进行电刺激对自由活动大鼠皮质酮分泌的影响。组织学检查显示电极位于紧邻CA1锥体细胞的位置。与假刺激对照组相比，接受高强度电刺激（1、10和100微安）的大鼠表现不同。它们更加活跃，并出现湿狗样抖动。接受不同电刺激强度的大鼠血浆皮质酮水平呈剂量依赖性增加。尽管预先用DSP4（60毫克/千克，腹腔注射）处理大鼠24小时可使海马去甲肾上腺素含量显著降低46%，但并未引起任何行为变化。DSP4处理对电刺激引起的皮质酮释放也没有影响。这些数据表明，刺激CA1锥体细胞可能导致皮质酮释放增加，并且海马去甲肾上腺素浓度的降低无法改变这种皮质酮反应。
