McDaniel T K, Jarvis K G, Donnenberg M S, Kaper J B
Department of Medicine, Department of Veterans Affairs Medical Center, Baltimore, MD.
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664-8. doi: 10.1073/pnas.92.5.1664.
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli O157:H7 are intestinal pathogens that profoundly damage the microvilli and subapical cytoskeleton of epithelial cells. Here we report finding in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions. DNA probes throughout this locus hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridize to avirulent members of the same species. The EPEC locus and a different virulence locus of uropathogenic E. coli insert into the E. coli chromosome at the identical site and share highly similar sequences near the point of insertion.
肠致病性大肠杆菌（EPEC）和肠出血性大肠杆菌O157:H7是肠道病原体，它们会严重破坏上皮细胞的微绒毛和顶端下细胞骨架。在此，我们报告在EPEC中发现了一个35千碱基对的基因座，其中包含几个与这些损伤形成有关的区域。该基因座上的DNA探针可与大肠杆菌O157:H7以及另外三个属中会造成类似损伤的其他病原体杂交，但不能与同一物种的无毒成员杂交。EPEC基因座和尿路致病性大肠杆菌的一个不同的毒力基因座在大肠杆菌染色体的同一位置插入，并在插入点附近共享高度相似的序列。
