Ayensu W K, Pucilowski O, Mason G A, Overstreet D H, Rezvani A H, Janowsky D S
Skipper Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill 27599.
Physiol Behav. 1995 Jan;57(1):165-9. doi: 10.1016/0031-9384(94)00204-i.
Complement proteins and fragments participate in the induction and modulation of specific and nonspecific immune reactions. We have examined the effect of 4 weeks of chronic mild stress (CMS) on complement sheep red blood cell hemolytic activity measured in CH50 units in two selectively bred lines of rats, the Flinders resistant line (FRL) and the Flinders sensitive line (FSL), that differ in cholinergic sensitivity and behavioral characteristics. Additionally, CMS-induced hedonic deficit (decreased preference for 0.02% saccharin over water) and serum corticosterone levels were compared in FRL and FSL rats. CMS caused a significantly (p < 0.01) greater decline in CH50 responses in FSL (-15%) than in FRL (-7%) rats. This was accompanied by a significant (p < 0.01) suppression of saccharin preference over a 24 h period in both FRL and FSL rats. Both lines showed a similar, more than 2-fold (p < 0.01) increase in corticosterone levels following CMS. These results further confirm that CMS induces a depressive-like state in rats as well as the validity of the FSL rat as a genetic model of depression. They also indicate that the effect of stress on the immune system can be monitored by measuring the complement CH50 response.
补体蛋白和片段参与特异性和非特异性免疫反应的诱导和调节。我们研究了4周慢性轻度应激（CMS）对两个选择性培育的大鼠品系（弗林德斯抗性系（FRL）和弗林德斯敏感系（FSL））中以CH50单位测量的补体绵羊红细胞溶血活性的影响，这两个品系在胆碱能敏感性和行为特征方面存在差异。此外，还比较了FRL和FSL大鼠中CMS诱导的享乐缺陷（对0.02%糖精的偏好低于水）和血清皮质酮水平。CMS导致FSL大鼠（-15%）的CH50反应下降幅度显著（p<0.01）大于FRL大鼠（-7%）。这伴随着FRL和FSL大鼠在24小时内对糖精偏好的显著（p<0.01）抑制。两个品系在CMS后皮质酮水平均出现相似的、超过2倍（p<0.01）的升高。这些结果进一步证实CMS在大鼠中诱导出类似抑郁的状态，以及FSL大鼠作为抑郁症遗传模型的有效性。它们还表明，通过测量补体CH50反应可以监测应激对免疫系统的影响。
