Kirchner J, Connolly C M, Sandmeyer S B
Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.
Science. 1995 Mar 10;267(5203):1488-91. doi: 10.1126/science.7878467.
The yeast retroviruslike element Ty3 inserts at the transcription initiation sites of genes transcribed by RNA polymerase III (Pol III). An in vitro integration assay was developed with the use of Ty3 viruslike particles and a modified SUP2 tyrosine transfer RNA (tRNA(Tyr)) gene target. Integration was position-specific and required Ty3 integrase, Pol III transcription factor (TF) IIIB-, TFIIIC-, and Pol III-containing fractions showed that TFIIIB and TFIIIC, together, were sufficient for position-specific Ty3 integration, but not for transcription. This report demonstrates that in vitro integration of a retroelement can be targeted by cellular proteins.
酵母逆转录病毒样元件Ty3插入由RNA聚合酶III（Pol III）转录的基因的转录起始位点。利用Ty3病毒样颗粒和修饰的SUP2酪氨酸转运RNA（tRNA(Tyr)）基因靶标开发了一种体外整合测定法。整合具有位置特异性，需要Ty3整合酶，含有Pol III转录因子（TF）IIIB、TFIIIC和Pol III的组分表明，TFIIIB和TFIIIC一起足以实现位置特异性Ty3整合，但不足以进行转录。本报告表明，逆转录元件的体外整合可被细胞蛋白靶向。
