Yao H, Wang F Z, Ding A S, Liu Z W, Ling S G
Institute of Basic Medical Science, Academy of Military Medical Science, Beijing.
Sheng Li Xue Bao. 1994 Dec;46(6):539-45.
The effects of recombinant human interleukin-1 beta (rhIL-1 beta) and interleukin-2 (rhIL-2) on the cultured rat hippocampal neurons were investigated with intracellular recording and extracellular micropressure ejection techniques. Experimental results showed that all of the neurons tested with rhIL-1 beta (100 U/ml) exhibited a 4.20 +/- 1.86 mV membrane hyperpolarization. 50% of the neurons treated with rhIL-2 (100 U/ml) showed a depolarization of 11.12 +/- 3.71 mV accompanied by bursting activity, while in a still higher concentration (1,000 U/ml) a 3.25 +/- 0.63 mV hyperpolarization was observed. All the above effects of rhIL-1 beta and rhIL-2 on the hippocampal neurons were completely reversible. The membrane resistance of the neurons was not significantly affected by these cytokines. These results suggest that the effects of cytokines IL-1 and IL-2 on the excitability of hippocampal neurons may be a way by which the immune system exerts a regulatory action on hippocampal function.
采用细胞内记录和细胞外微压喷射技术，研究了重组人白细胞介素 -1β（rhIL -1β）和白细胞介素 -2（rhIL -2）对培养的大鼠海马神经元的影响。实验结果表明，所有用rhIL -1β（100 U/ml）处理的神经元均表现出4.20±1.86 mV的膜超极化。50%用rhIL -2（100 U/ml）处理的神经元表现出11.12±3.71 mV的去极化并伴有爆发性活动，而在更高浓度（1000 U/ml）时观察到3.25±0.63 mV的超极化。rhIL -1β和rhIL -2对海马神经元的上述所有作用都是完全可逆的。这些细胞因子对神经元的膜电阻没有显著影响。这些结果表明，细胞因子IL -1和IL -2对海马神经元兴奋性的影响可能是免疫系统对海马功能发挥调节作用的一种方式。
