使用带氮磷检测器的毛细管气液色谱法快速同时测定利多卡因、布比卡因及其两种主要代谢物。

Rapid simultaneous determination of lidocaine, bupivacaine, and their two main metabolites using capillary gas-liquid chromatography with nitrogen phosphorus detector.

作者信息

Lorec A M, Bruguerolle B, Attolini L, Roucoules X

机构信息

Medical and Clinical Pharmacology Laboratory, Faculty of Medicine of Marseille, France.

出版信息

Ther Drug Monit. 1994 Dec;16(6):592-5. doi: 10.1097/00007691-199412000-00010.

DOI:10.1097/00007691-199412000-00010

PMID:7878699

Abstract

A gas-liquid chromatographic method for the simultaneous measurement in serum of bupivacaine, lidocaine, and their main metabolites, 2,6-pipecolylxylidide (PPX) and monoethylglycine xylidide (MEGX), respectively, is described. The procedure involves a one-step extraction and injection of the extract into a gas chromatograph equipped with a capillary column and nitrogen phosphorus detector under constant temperature conditions. Recovery of all components averaged 94%, with the lowest detection limit of 15 ng/ml for the four drugs. The precision within-series coefficients of variation ranged from 7.7% for bupivacaine, 8.6% for lidocaine, 10.2% for MEGX, and 15.8% for PPX. The interday coefficients ranged from 0.7 to 6.5%. Concomitant use of caffeine and carbamazepine may interfere with MEGX and bupivacaine determination, respectively. For this reason, in patients receiving one of these two drugs (or ingesting foods and beverages containing caffeine), the described method is not recommended.

摘要

本文描述了一种气相色谱法，用于同时测定血清中的布比卡因、利多卡因及其主要代谢物，即分别为2,6-哌啶基二甲苯胺（PPX）和单乙基甘氨酸二甲苯胺（MEGX）。该方法包括一步萃取，并将萃取物注入配备毛细管柱和氮磷检测器的气相色谱仪中，在恒温条件下进行分析。所有组分的回收率平均为94%，四种药物的最低检测限为15 ng/ml。日内变异系数范围为：布比卡因为7.7%，利多卡因为8.6%，MEGX为10.2%，PPX为15.8%。日间系数范围为0.7%至6.5%。同时使用咖啡因和卡马西平可能分别干扰MEGX和布比卡因的测定。因此，对于正在服用这两种药物之一（或摄入含有咖啡因的食物和饮料）的患者，不推荐使用本文所述方法。