Burm A G, van Kleef J W, de Boer A G
Anesthesiology. 1982 Dec;57(6):527-9. doi: 10.1097/00000542-198212000-00015.
A capillary gas chromatographic method for the quantitative determination of bupivacaine in plasma is described. Bupivacaine and an added internal standard were extracted from plasma with n-pentane. The extracted products were introduced into the gas chromatograph via a solid injection system. The combination of a support coated open tubular column and a nitrogen-selective detector insure a good selectivity and a high sensitivity. The coëfficient of variation at concentrations exceeding 3 ng bupivacaine/ml plasma is less than 6%. The detection limit is about 1 ng/ml.
本文描述了一种用于定量测定血浆中布比卡因的毛细管气相色谱法。布比卡因和添加的内标物用正戊烷从血浆中萃取。萃取产物通过固体进样系统引入气相色谱仪。载体涂渍空心柱和氮选择性检测器的组合确保了良好的选择性和高灵敏度。布比卡因浓度超过3 ng/ml血浆时的变异系数小于6%。检测限约为1 ng/ml。
