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Comparison of the reactions of chemically reactive analogs of U-G-A and of A-U-G with ribosomes of Escherichia coli.

作者信息

Pongs O, Rossner E

出版信息

Nucleic Acids Res. 1976 Jul;3(7):1625-33. doi: 10.1093/nar/3.7.1625.

DOI:10.1093/nar/3.7.1625
PMID:787925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343023/
Abstract

The chemically reactive analog of U-G-A, 5'-(4-(Bromo-[2-14C] acetamido) phenylphospho) - uridylyl-(3'-5') - guanylyl-(3'-5') adenosine has a 20 fold lower affinity to 70S ribosomes than the corresponding analog of A-U-G though the U-G-A analog also preferentially reacts with protein S18 of 70S ribosomes. This reaction programs ribosomes for EF-T dependent Trp-tRNATrp-suIII binding. Therefore, it is concluded that this protein is part of the A'-site of the ribosomal codon binding site. Reaction of the U-G-A analog with 30S subunits lead to a predominant crosslinking of U-G-A to proteins S4 and S18. In contrast, a comparable reaction of the A-U-G analog with 30S subunits lead to a predominant crosslinking of A-U-G to proteins S4 and S12 (Pongs, O., Stoffler, G.A., Lanka, E., (1975) J. Mol. Biol. 99, 301). Since protein S12 is located at the 'P' site of the ribosomal codon binding site, it is proposed that the U-G-A analog does not bind at this site.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e9/343023/09932deefd81/nar00492-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e9/343023/6bf865053840/nar00492-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e9/343023/09932deefd81/nar00492-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e9/343023/6bf865053840/nar00492-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e9/343023/09932deefd81/nar00492-0012-a.jpg

相似文献

1
Comparison of the reactions of chemically reactive analogs of U-G-A and of A-U-G with ribosomes of Escherichia coli.
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引用本文的文献

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Nucleic Acids Res. 1978 Sep;5(9):3389-407. doi: 10.1093/nar/5.9.3389.
3
Analogs of methionyl-tRNA synthetase substrates containing photolabile groups.含有光不稳定基团的甲硫氨酰 - tRNA合成酶底物类似物。

本文引用的文献

1
The relationship between poly A polymerase and the ribosomes.
Biochemistry. 1966 Nov;5(11):3676-84. doi: 10.1021/bi00875a042.
2
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.大肠杆菌16S核糖体RNA的3'末端序列：与无义三联体及核糖体结合位点的互补性
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6. doi: 10.1073/pnas.71.4.1342.
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On the structure of yeast tRNA Phe . Complementary-oligonucleotide binding studies.
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Use of computerized multidimensional scaling to compare immunoelectron microscopy data with protein near-neighbor information: application to the 30S ribosome from Escherichia coli.使用计算机化多维标度法将免疫电子显微镜数据与蛋白质近邻信息进行比较：应用于大肠杆菌的30S核糖体。
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3128-32. doi: 10.1073/pnas.75.7.3128.
Molecular interactions of ribosomal components. 3. Isolation of the RNA binding site for a ribosomal protein.
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5
An improved technique for the analysis of ribonucleoprotein fragments from Escherichia coli 30S ribosomes.一种用于分析大肠杆菌30S核糖体核糖核蛋白片段的改进技术。
Eur J Biochem. 1971 Nov 11;23(1):52-60. doi: 10.1111/j.1432-1033.1971.tb01591.x.
6
30S ribosomal proteins associated with the 3'-terminus of 16S RNA.
FEBS Lett. 1975 Oct 15;58(1):281-4. doi: 10.1016/0014-5793(75)80279-1.
7
Synthesis of a chemically reactive analog of the nonsense codon U-G-A. Its reaction with ribosomes of Escherichia coli.
Hoppe Seylers Z Physiol Chem. 1975 Aug;356(8):1297-304. doi: 10.1515/bchm2.1975.356.2.1297.
8
Synthesis of a chemically reactive analog of the initiation codon: its reaction with ribosomes of Escherichia coli.起始密码子的化学反应性类似物的合成：其与大肠杆菌核糖体的反应
Hoppe Seylers Z Physiol Chem. 1975 Apr;356(4):449-58. doi: 10.1515/bchm2.1975.356.1.449.
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Photoaffinity reaction between polyuridylic acid and protein S1 on the Escherichia coli ribosome.
FEBS Lett. 1975 Apr 1;52(2):281-3. doi: 10.1016/0014-5793(75)80825-8.
10
Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.用AUG底物类似物对核糖体解码位点进行亲和标记。
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