大肠杆菌RNA聚合酶向不依赖模板的酶的转变。
Conversion of Escherichia coli RNA polymerase to a template independent enzyme.
作者信息
Berge R K, Haarr L, Nygaard A P
出版信息
Nucleic Acids Res. 1976 Aug;3(8):1937-45. doi: 10.1093/nar/3.8.1937.
Abstract
Preparations of RNA polymerase (E.C.2.7.7.6) from uninfected Escherichia coli, T4 infected Escherichia coli, and Acinetobacter calcoaceticus when centrifuged in sucrose gradients in the absence of magnesium ions gave rise to five peaks, all of which were able to form polymers from ribonucleoside 5'-triphosphates in the absence of template or primer. All of the peaks obtained from the Escherichia coli enzyme appeared to contain the subunit alpha and beta and, in addition, polypeptides which appeared to be derived from the subunit beta.
摘要
从未感染的大肠杆菌、T4感染的大肠杆菌以及乙酸钙不动杆菌中制备的RNA聚合酶（E.C.2.7.7.6），在不含镁离子的情况下于蔗糖梯度中离心时会产生五个峰，所有这些峰在没有模板或引物的情况下都能够从核糖核苷5'-三磷酸形成聚合物。从大肠杆菌酶获得的所有峰似乎都含有α和β亚基，此外，还有似乎源自β亚基的多肽。
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