大肠杆菌RNA聚合酶的动力学
The kinetics of E. coli RNA polymerase.
作者信息
Solage A, Cedar H
出版信息
Nucleic Acids Res. 1976 Sep;3(9):2207-22. doi: 10.1093/nar/3.9.2207.
Abstract
Using an assay specific for chain elongation of E. coli RNA polymerase the kinetics of this propagation reaction have been studied. The kinetic behaviour is consistent woth the mathematical model formulated for this multisubstrate enzyme. The effect of increasing salt concentration on the kinetics of the reaction indicated that DNA unwinding is probably a necessary step in the propagation step, although this may not be the rate limiting step under all conditions.
摘要
利用一种针对大肠杆菌RNA聚合酶链延伸的测定方法，对该延伸反应的动力学进行了研究。动力学行为与针对这种多底物酶建立的数学模型一致。增加盐浓度对反应动力学的影响表明，DNA解旋可能是延伸步骤中的一个必要步骤，尽管在所有条件下这可能不是限速步骤。
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