大肠杆菌RNA聚合酶与染色质亚基的结合。
Binding of E. coli RNA polymerase to chromatin subunits.
作者信息
Bustin M
出版信息
Nucleic Acids Res. 1978 Mar;5(3):925-32. doi: 10.1093/nar/5.3.925.
Abstract
Chromatin subunits were prepared from purified rat liver nuclei and the template properties of the nucleosome preparation studied. It was found that: 1) The fundamental template restriction of chromatin (as compared to deproteinized DNA) is retained in the isolated nucleosomes, 2) On the average one molecule of RNA polymerase is bound to one molecule of DNA purified from nucleosomes, 3) The number of RNA polymerase binding sites on chromatin subunits is 6 to 20 times lower than that of the DNA extracted from these subunits, 4) Transcription can proceed through nucleosomes resulting in RNA chains approximately 150 nucleotides long.
摘要
从纯化的大鼠肝细胞核中制备染色质亚基，并研究了核小体制剂的模板特性。结果发现：1）染色质的基本模板限制（与脱蛋白DNA相比）在分离的核小体中得以保留；2）平均而言，一个RNA聚合酶分子与从核小体中纯化的一个DNA分子结合；3）染色质亚基上RNA聚合酶结合位点的数量比从这些亚基中提取的DNA低6至20倍；4）转录可以通过核小体进行，产生约150个核苷酸长的RNA链。
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本文引用的文献
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Transcription of chromatin in vitro.体外染色质转录
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