Metzner B, Elsner J, Dobos G, Kownatzki E, Parlow F, Schraufstätter I, Norgauer J
University of Freiburg, Department of Dermatology, Germany.
Agents Actions. 1994 Oct;42(3-4):101-6. doi: 10.1007/BF01983473.
The neutrophil chemotaxins, complement fragment C5a (C5a) and GRO alpha, induced the mobilization of Ca2+ from intracellular stores and the polymerization of actin in human neutrophils as assayed by flow cytometric measurements. [Ca2+]i-transients developed as an "all-or-none" response. Individual neutrophils required different threshold concentrations of added ligand to induce [Ca2+]i-transients which were then always maximal. In contrast, chemotaxin-induced formation of actin filaments in single neutrophils occurred in a dose-dependent manner. Pertussis toxin blocked chemotaxin-induced actin polymerization and [Ca2+]i-transients indicating that both cell responses shared initial activation steps such as ligand binding and activation of guanine nucleotide-binding proteins (G-proteins).
中性粒细胞趋化因子、补体片段C5a（C5a）和GROα，通过流式细胞术检测发现，可诱导人中性粒细胞内储存钙的动员以及肌动蛋白的聚合。胞内钙瞬变以“全或无”反应形式出现。单个中性粒细胞诱导胞内钙瞬变需要不同阈值浓度的添加配体，且一旦诱导，钙瞬变总是最大程度的。相反，趋化因子诱导单个中性粒细胞中肌动蛋白丝的形成呈剂量依赖性。百日咳毒素可阻断趋化因子诱导的肌动蛋白聚合和胞内钙瞬变，表明这两种细胞反应共享诸如配体结合和鸟嘌呤核苷酸结合蛋白（G蛋白）激活等初始激活步骤。
