Huggins C B, Anderson K P
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3283-7. doi: 10.1073/pnas.73.9.3283.
The tails of newborn rats, consisting of slender continuous cones of avascular cartilage, were transplanted in subcutaneous spaces of allogeneic adolescents; colloidal carbon was injected in a vein before the transplants were harvested on days 7-28. Between 97 and 100% of the transplants were accepted, underwent differentiation into bone with bone marrow, and grew at a brisk rate. Acceptance was recognized by (i) a zebra-stripe effect, visible in the gross, resulting from accumulation of carbon in reticuloendothelial cells; (ii) increase of alkaline phosphatase [or-thophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] and of incorporated 45Ca in the transplant; and (iii) the presence of dense cortical bone with lacunae populated with osteocytes demonstrable by histology. Differentiation of the cartilaginous transplant into bone with subsequent growth of the transplant occurred in recipients fed sucrose as their sole ration.
新生大鼠的尾巴由细长连续的无血管软骨锥体组成，被移植到同种异体青少年的皮下间隙；在第7 - 28天收获移植组织前，经静脉注射胶体碳。97%至100%的移植组织被接受，分化为带有骨髓的骨组织，并快速生长。移植组织被接受可通过以下几点来确认：(i) 肉眼可见的斑马条纹效应，这是由于碳在网状内皮细胞中积聚所致；(ii) 移植组织中碱性磷酸酶[正磷酸单酯磷酸水解酶（最适pH为碱性），EC 3.1.3.1]和掺入的45Ca增加；(iii) 组织学显示存在有骨陷窝且充满骨细胞的致密皮质骨。以蔗糖作为唯一食物的受体中，软骨移植组织会分化为骨组织并随后生长。
