伴有β2-糖蛋白I依赖性抗磷脂抗体的系统性红斑狼疮患者中β2-糖蛋白I抗体的患病率。
Prevalence of beta 2-glycoprotein I antibody in systemic lupus erythematosus patients with beta 2-glycoprotein I dependent antiphospholipid antibodies.
作者信息
Matsuda J, Saitoh N, Gotoh M, Gohchi K, Tsukamoto M
机构信息
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
出版信息
Ann Rheum Dis. 1995 Jan;54(1):73-5. doi: 10.1136/ard.54.1.73.
OBJECTIVE
To investigate whether beta 2-glycoprotein I antibody (aGPI) exists in sera that are positive for antiphospholipid antibodies (aPL).
METHODS
Thirty six patients with systemic lupus erythematosus (SLE) and 20 healthy volunteers were recruited. GPI independent and dependent aPL (anticardiolipin (aCL), antiphosphatidylserine, antiphosphatidylinositol, antiphosphatidic acid) were measured using a conventional enzyme linked immunosorbent assay (ELISA). aGPI was measured by an ELISA using a gamma irradiated plate coated with purified GPI.
RESULTS
Twenty of 36 SLE patients were positive for at least one of the GPI-dependent aPL. Five of these 20 were also positive for aGPI. Another five of the 20 showed low GPI dependency aCL antibodies; however, three of these patients required GPI for binding to phosphatidylserine, phosphatidylinositol, or both.
CONCLUSION
We confirmed the existence of aGPI in patients with true GPI dependent aPL. From these results, we recommend the use of a gamma irradiated plate with GPI as antigen for the detection of true GPI dependent aPL or aGPI.
目的
研究抗磷脂抗体（aPL）阳性血清中是否存在β2-糖蛋白I抗体（aGPI）。
方法
招募了36例系统性红斑狼疮（SLE）患者和20名健康志愿者。使用传统酶联免疫吸附测定（ELISA）检测与GPI无关和相关的aPL（抗心磷脂（aCL）、抗磷脂酰丝氨酸、抗磷脂酰肌醇、抗磷脂酸）。通过使用涂有纯化GPI的γ射线照射板的ELISA检测aGPI。
结果
36例SLE患者中有20例至少一种与GPI相关的aPL呈阳性。这20例中的5例aGPI也呈阳性。这20例中的另外5例显示低GPI依赖性aCL抗体；然而，这些患者中有3例需要GPI才能与磷脂酰丝氨酸、磷脂酰肌醇或两者结合。
结论
我们证实了真正的GPI依赖性aPL患者中存在aGPI。根据这些结果，我们建议使用涂有GPI的γ射线照射板作为抗原，用于检测真正的GPI依赖性aPL或aGPI。
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