García-Sancho J, Montero M, Alvarez J, Fonteriz R I, Sanchez A
Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Universidad de Valladolid, Spain.
Bioelectromagnetics. 1994;15(6):579-88. doi: 10.1002/bem.2250150611.
We have investigated the effects of sinusoidal electromagnetic fields (EMF) on ion transport (Ca2+, Na+, K+, and H+) in several cell types (red blood cells, thymocytes, Ehrlich ascites tumor cells, and HL60 and U937 human leukemia cells). The effects on the uptake of radioactive tracers as well as on the cytosolic Ca2+ concentration ([Ca2+]i), the intracellular pH (pHi), and the transmembrane potentsial (TMP) were studied. Exposure to EMF at 50 Hz and 100-2000 microT (rms) had no significant effects on any of these parameters. Exposure to EMF of 20-1200 microT (rms) at the estimated cyclotron magnetic resonance frequencies for the respective ions had no significant effects except for a 12-32% increase of the uptake of 42K within a window at 14.5-15.5 Hz and 100-200 microT (rms), which was found in U937 and Ehrlich cells but not in the other cell types.
我们研究了正弦电磁场（EMF）对几种细胞类型（红细胞、胸腺细胞、艾氏腹水肿瘤细胞以及HL60和U937人白血病细胞）中离子转运（Ca2+、Na+、K+和H+）的影响。研究了其对放射性示踪剂摄取以及胞质Ca2+浓度（[Ca2+]i）、细胞内pH值（pHi）和跨膜电位（TMP）的影响。暴露于50Hz和100 - 2000微特斯拉（均方根值）的电磁场对这些参数均无显著影响。在各个离子的估计回旋共振频率下，暴露于20 - 1200微特斯拉（均方根值）的电磁场除了在14.5 - 15.5Hz和100 - 200微特斯拉（均方根值）范围内U937细胞和艾氏细胞中42K摄取增加12 - 32%外，对其他参数均无显著影响，而在其他细胞类型中未发现此现象。
