Semiletov Iu A, Karpova V A, Kalinina T I, Khudiakov Iu E
Bioorg Khim. 1994 Nov;20(11):1175-85.
Based on theoretical analysis of secondary structure and hydrophilicity data, the region (residues 73-89) of the HBV core-antigen presumably containing the antigenic determinant has been revealed. The epitope mapping of this region with the use of synthetic peptides, obtained by the pin technology and solid phase method, was carried out. Peptides were synthesized in two variants with different amino acids residues in the position 80 (Ala, Ile). Free peptides and their conjugates with bovine serum albumin were tested for antigenicity in ELISA. The results indicate that the fragment 78-83 is the shortest region of the core-antigen which reacts with antibodies from hepatitis B patients sera.
基于对二级结构和亲水性数据的理论分析，已揭示出乙肝病毒核心抗原中可能包含抗原决定簇的区域（第73 - 89位氨基酸残基）。利用通过针技术和固相法获得的合成肽对该区域进行了表位作图。肽以两种变体形式合成，在第80位氨基酸残基处具有不同的氨基酸（丙氨酸、异亮氨酸）。在酶联免疫吸附测定（ELISA）中测试了游离肽及其与牛血清白蛋白的缀合物的抗原性。结果表明，片段78 - 83是核心抗原中与乙肝患者血清抗体发生反应的最短区域。
