Semiletov Iu A, Karpova V A, Smirnov V D, Viazov S O
Bioorg Khim. 1993 Mar;19(3):277-85.
A set of 8 peptides from the immunodominant region (65-80 aa) of delta-antigen was prepared by solid-phase synthesis. Peptide 71-80 was synthesized in two variants--with different amino acid residues in positions 73, 74 and 76. Free peptides and their conjugates with bovine serum albumin were tested for antigenicity in ELISA. The correlation between the peptide chain's length and its antigenic activity was noted. Peptides 65-80 and 69-80 displayed a positive reaction with all individual sera and pools of sera from HDV chronic patients. Both variants of the peptide 71-80 reacted with 100% of sera pools but only with 83% of individual sera. Smaller peptides from the same 65-80 region (73-80, 69-78, 71-78, 71-76) did not bind to any anti-delta positive serum. All synthesized peptides reacted strongly with rabbit antisera raised to the conjugate of peptide 65-80 with bovine albumin. These findings suggest that delta-antigen contains multiple highly immunogenic epitopes associated with the single immunodominant site between 69 and 80 amino acid residues.
通过固相合成制备了一组来自δ抗原免疫显性区域（65 - 80个氨基酸）的8种肽。肽71 - 80以两种变体形式合成，在第73、74和76位具有不同的氨基酸残基。对游离肽及其与牛血清白蛋白的缀合物进行了ELISA抗原性检测。观察到肽链长度与其抗原活性之间的相关性。肽65 - 80和69 - 80与HDV慢性患者的所有个体血清和血清池均呈阳性反应。肽71 - 80的两种变体均与100%的血清池反应，但仅与83%的个体血清反应。来自相同65 - 80区域的较小肽（73 - 80、69 - 78、71 - 78、71 - 76）不与任何抗δ阳性血清结合。所有合成肽均与针对肽65 - 80与牛白蛋白缀合物产生的兔抗血清强烈反应。这些发现表明，δ抗原含有多个与69至80个氨基酸残基之间的单个免疫显性位点相关的高度免疫原性表位。
