Barnea E R, Lahijani K I, Roussev R, Barnea J D, Coulam C B
Reproductive Immunology, Genetics & IVF Institute, Fairfax, VA 22031.
Am J Reprod Immunol. 1994 Oct;32(3):133-8. doi: 10.1111/j.1600-0897.1994.tb01103.x.
To evaluate the ability of the lymphocyte/platelet binding assay to identify a preimplantation factor (PIF).
Percentages of binding of lymphocytes by platelets in the presence of sera from 30 known pregnant and 30 nonpregnant individuals were compared using a novel lymphocyte/platelet binding assay. The assay is performed using a combination of a heat inactivated sera with donor O+lymphocytes, activated complement and an antibody against CD2 (T11, Ortho Pharmaceuticals).
In nonpregnant females (23.6 +/- 6.5%) and males (17.7 +/- 4.7%) the percentage of lymphocytes bound by platelets was significantly different from pregnant women (56.1 +/- 15.9%) (P < 0.0001). Serial sampling of blood in five women undergoing IVF/ET who had normal pregnancies showed the detection of PIF by 4 days after transfer. The lymphocyte/platelet binding assay was not influenced by hCG, progesterone and estradiol. The interassay and intrassay variabilities were < 3%.
The lymphocyte/platelet binding assay is a simple, reproducible, specific and cost efficient assay for measurement of PIF. Application of this assay will provide investigative and diagnostic tools for identifying and monitoring early pregnancy events.
评估淋巴细胞/血小板结合试验识别着床前因子（PIF）的能力。
使用一种新型淋巴细胞/血小板结合试验，比较30名已知怀孕个体和30名未怀孕个体血清存在时血小板与淋巴细胞的结合百分比。该试验采用热灭活血清与供体O+淋巴细胞、活化补体及抗CD2抗体（T11，Ortho制药公司）联合进行。
未怀孕女性（23.6±6.5%）和男性（17.7±4.7%）中，血小板结合的淋巴细胞百分比与孕妇（56.1±15.9%）有显著差异（P<0.0001）。对5名接受体外受精/胚胎移植且妊娠正常的女性进行血液连续采样，结果显示移植后4天可检测到PIF。淋巴细胞/血小板结合试验不受人绒毛膜促性腺激素、孕酮和雌二醇的影响。试验间和试验内变异均<3%。
淋巴细胞/血小板结合试验是一种用于测量PIF的简单、可重复、特异且经济高效的试验。该试验的应用将为识别和监测早期妊娠事件提供研究和诊断工具。
