肿瘤坏死因子-α（TNF-α）和肿瘤坏死因子受体p55（TNFR p55）在培养的羊膜绒毛膜中的表达。
Expression of TNF-alpha and TNFR p55 in cultured amniochorion.
作者信息
Fortunato S J, Menon R, Swan K F
机构信息
Department of Obstetrics and Gynecology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
出版信息
Am J Reprod Immunol. 1994 Oct;32(3):188-93. doi: 10.1111/j.1600-0897.1994.tb01113.x.
PROBLEM
Preterm labor and PROM are major complications of pregnancy. We have reported the possible role of amniochorionic membrane as it relates to the production of cytokines and the early onset of labor. Amniochorion is capable of responding to an infectious process with the production of IL-6 and IL-1 beta. Here we examine the expression of TNF-alpha and TNFR in amniochorion.
METHOD
Amniochorionic membranes were collected and maintained in an organ explant system. Samples were frozen and/or fixed for RT-PCR, in situ hybridization, and immunocytochemistry.
RESULTS
RT-PCR demonstrated mRNA for TNF-alpha and in situ hybridization localized mRNA in chorion and amnion. Immunocytochemistry demonstrated TNF-alpha peptide in amnion but not in chorion. Immunocytochemical localization of TNFR indicates presence of that peptide in both amnion and chorion.
CONCLUSIONS
We conclude that the fetal membranes are sources of TNF-alpha and TNFR, supporting our previous work indicating that fetal membranes are active participants in the response to intraamniotic infection.
问题
早产和胎膜早破是妊娠的主要并发症。我们已经报道了羊膜绒毛膜与细胞因子产生及分娩早期发作相关的可能作用。羊膜绒毛膜能够通过产生白细胞介素-6和白细胞介素-1β来应对感染过程。在此，我们检测肿瘤坏死因子-α（TNF-α）和肿瘤坏死因子受体（TNFR）在羊膜绒毛膜中的表达。
方法
收集羊膜绒毛膜并维持在器官外植体系统中。样本经冷冻和/或固定后用于逆转录聚合酶链反应（RT-PCR）、原位杂交和免疫细胞化学检测。
结果
RT-PCR显示存在TNF-α的信使核糖核酸（mRNA），原位杂交将mRNA定位在绒毛膜和羊膜中。免疫细胞化学显示TNF-α肽存在于羊膜中，但不存在于绒毛膜中。TNFR的免疫细胞化学定位表明该肽在羊膜和绒毛膜中均有存在。
结论
我们得出结论，胎膜是TNF-α和TNFR的来源，支持我们之前的研究工作，即胎膜是羊膜腔内感染反应的积极参与者。
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