羊膜绒毛膜：白细胞介素-8的一个来源。
Amniochorion: a source of interleukin-8.
作者信息
Fortunato S J, Menon R, Swan K F
机构信息
Department of Maternal-Fetal Medicine, Women's Hospital, Centennial Medical Center, Nashville, Tennessee, USA.
出版信息
Am J Reprod Immunol. 1995 Sep;34(3):156-62. doi: 10.1111/j.1600-0897.1995.tb00932.x.
PROBLEM
Preterm labor and premature rupture of the membranes are major complications of pregnancy. We have reported the possible role of amniochorionic membrane in the production of inflammatory cytokines and the early onset of labor. This study was conducted to detect the expression of IL-8 mRNA and peptide production in cultured fetal membranes.
METHOD
Amniochorionic membranes were collected from women undergoing elective cesarean section at term. Membranes were cultured in an organ explant system and the expression of IL-8 was studied over a 10-day period by RT-PCR and in situ hybridization. IL-8 peptide localization was accomplished using immunocytochemistry.
RESULTS
Constitutive expression of IL-8 mRNA in cultured fetal membranes was demonstrated in both amniotic and chorionic leave cells. mRNA and peptide for IL-8 was homogeneously distributed throughout the amniotic and chorionic cells.
CONCLUSION
Human amniochorionic membrane is a source of IL-8 mRNA and peptide.
问题
早产和胎膜早破是妊娠的主要并发症。我们已经报道了羊膜绒毛膜在炎性细胞因子产生和分娩早期发作中的可能作用。本研究旨在检测培养的胎膜中白细胞介素-8（IL-8）信使核糖核酸（mRNA）的表达及肽的产生。
方法
从足月行择期剖宫产的妇女中收集羊膜绒毛膜。将胎膜在器官外植体系统中培养，并通过逆转录聚合酶链反应（RT-PCR）和原位杂交在10天的时间内研究IL-8的表达。使用免疫细胞化学法完成IL-8肽的定位。
结果
在培养的胎膜中，羊膜和绒毛膜细胞均显示出IL-8 mRNA的组成性表达。IL-8的mRNA和肽在整个羊膜和绒毛膜细胞中均匀分布。
结论
人羊膜绒毛膜是IL-8 mRNA和肽的来源。
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