羊膜绒毛膜的体外器官培养
Organ culture of amniochorionic membrane in vitro.
作者信息
Fortunato S J, Menon R, Swan K F, Lyden T W
机构信息
Department of Obstetrics and Gynecology, Tulane University School of Medicine, New Orleans, Louisiana 70112.
出版信息
Am J Reprod Immunol. 1994 Oct;32(3):184-7. doi: 10.1111/j.1600-0897.1994.tb01112.x.
PROBLEM
The purpose of the study was to develop a novel method of amniochorionic membrane culture aimed at maintaining tissue integrity.
METHOD
Amniochorionic membranes were collected from women prior to labor, undergoing elective cesarean section, with no history of infection or pregnancy related complication. Fetal membranes were maintained in culture for up to ten days. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a "house keeping" gene and inflammatory cytokine mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) at various periods of culture. mRNA localization was performed by in situ hybridization.
RESULTS
GAPDH gene expression was seen throughout the culture period. Inflammatory cytokine mRNA (IL-1 alpha, IL-1 beta and IL-6) were also detected during culture. Cellular and tissue morphology appeared normal.
CONCLUSIONS
The culture technique we propose is a simple organ explant system which maintains the morphology and autocrine/paracrine relationships within this tissue.
问题
本研究的目的是开发一种旨在维持组织完整性的新型羊膜绒毛膜培养方法。
方法
从择期剖宫产的未临产女性中收集羊膜绒毛膜，这些女性无感染史或妊娠相关并发症。将胎膜培养长达十天。在培养的不同阶段，通过逆转录聚合酶链反应（RT-PCR）检测甘油醛-3-磷酸脱氢酶（GAPDH）（一种“管家”基因）和炎性细胞因子mRNA。通过原位杂交进行mRNA定位。
结果
在整个培养期间均可见GAPDH基因表达。培养期间也检测到炎性细胞因子mRNA（IL-1α、IL-1β和IL-6）。细胞和组织形态正常。
结论
我们提出的培养技术是一种简单的器官外植体系统，可维持该组织内的形态以及自分泌/旁分泌关系。
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