Akiba T, Nishi A, Takaoki M, Nagaoka S, Tomita F
Institute of Physical and Chemical Research, Saitama, Japan.
Appl Theor Electrophor. 1994;4(2):65-9.
Electrophoretic free mobilities (EFM) of four fungal spores and five bacterial cells were determined in 7 mM triethanolamine/acetate (TEA) buffer by means of microscopic electrophoresis (ME) and free flow electrophoresis (FFE). Spores of Aspergillus terreus, Penicillium citrinum, Gliocladium virens, and Rhizopus oryzae had similar EFM from 2.2 to 3.1 microns sec-1/V cm-1. The resolution of the spore mixture by FFE was therefore determined to be poor. Bacterial cells of Salmonella typhimurium LT2 mutants showed a distinctive EFM from 0 to 4.2 microns sec-1/V cm-1, which is a large enough difference to produce a clear separation of each mutant type from the mixture in FFE. The differences in the EFM of bacteria result from defective structures in the lipopolysaccharide of their outer membranes. The viability of bacteria in TEA buffer at 4 degrees C was investigated, and it was found to be stable for 14 days. This period is long enough to allow the performance of space experiments.
通过显微镜电泳（ME）和自由流动电泳（FFE），在7 mM三乙醇胺/乙酸盐（TEA）缓冲液中测定了四种真菌孢子和五种细菌细胞的电泳自由迁移率（EFM）。土曲霉、桔青霉、绿粘帚霉和米根霉的孢子具有相似的EFM，范围为2.2至3.1微米秒⁻¹/伏厘米⁻¹。因此，FFE对孢子混合物的分离效果较差。鼠伤寒沙门氏菌LT2突变体的细菌细胞显示出独特的EFM，范围为0至4.2微米秒⁻¹/伏厘米⁻¹，这种差异足够大，能够在FFE中从混合物中清晰分离出每种突变体类型。细菌EFM的差异源于其外膜脂多糖结构的缺陷。研究了细菌在4℃的TEA缓冲液中的活力，发现其在14天内保持稳定。这段时间足够长，可以进行空间实验。
