Shahan T A, Sorenson W G, Lewis D M
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505.
Environ Res. 1994 Oct;67(1):98-107. doi: 10.1006/enrs.1994.1067.
High amounts of fungal spores, bacteria, and bacterial endotoxin have been found in dust associated with the poorly characterized syndrome, organic dust toxic syndrome (ODTS). As part of an ongoing investigation to determine the etiopathogenesis for ODTS, this study has focused on activation of guinea pig bronchial alveolar lavage (BAL) cells as evidenced by the production of superoxide anion in response to fungal spores and lipopolysaccharide (LPS). Fungal spores from Aspergillus candidus, Aspergillus terreus, Aspergillus niger, Aspergillus fumigatus, Eurotima amstelodami, Penicillium spinulosum, and Cladosporium cladosporioides were all shown to increase superoxide anion production, each with different potencies. LPS stimulated little superoxide anion production in BAL cells, but when cells were pretreated with LPS prior to stimulation with fungal spores, superoxide anion production was increased over that induced by either spores or LPS alone. These results suggest that the inhalation of LPS together with fungal spores could possibly provoke abnormal lung pathologies.
在与特征不明的有机粉尘中毒综合征（ODTS）相关的灰尘中，已发现大量真菌孢子、细菌和细菌内毒素。作为确定ODTS病因发病机制的一项正在进行的调查的一部分，本研究聚焦于豚鼠支气管肺泡灌洗（BAL）细胞的激活，这可通过对真菌孢子和脂多糖（LPS）产生超氧阴离子来证明。来自白色念珠菌、土曲霉、黑曲霉、烟曲霉、阿姆斯特丹散囊菌、棘孢青霉和枝孢霉的真菌孢子均显示可增加超氧阴离子的产生，每种孢子的效力不同。LPS在BAL细胞中刺激产生的超氧阴离子很少，但当细胞在用真菌孢子刺激之前先用LPS预处理时，超氧阴离子的产生比单独由孢子或LPS诱导的增加。这些结果表明，吸入LPS和真菌孢子可能会引发异常的肺部病变。
