Robertson J S
National Institute for Biological Standards and Control, Potters Bar, UK.
Dev Biol Stand. 1994;83:81-5.
For sequence analysis of transfected genes there are two approaches which can be adopted. One is to derive a consensus sequence of the gene in question and this may, in addition to providing the basic sequence, provide limited information on the presence of variant sequences. In order to provide more extensive data on variant sequences a second approach has to be adopted and that is to subclone the transfected gene (or mRNA) and analyse the sequences of large numbers of the subclones. While there are few obstacles nowadays to deriving a consensus sequence, considerable care has to be exercised in interpreting sequence data derived after subcloning because of the possible introduction of laboratory artefacts.
对于转染基因的序列分析，可以采用两种方法。一种是推导所研究基因的共有序列，这除了能提供基本序列外，还可能提供关于变异序列存在情况的有限信息。为了获得关于变异序列更广泛的数据，必须采用第二种方法，即对转染基因（或mRNA）进行亚克隆，并分析大量亚克隆的序列。如今推导共有序列几乎没有什么障碍，但由于可能引入实验室假象，在解释亚克隆后获得的序列数据时必须格外小心。
