Brudnak M, Miller K S
Faculty of Biological Science, University of Tulsa, OK 74104.
Biotechniques. 1993 Jan;14(1):66-8.
Expression cloning in eukaryotic cells has allowed the isolation of many genes for which no protein or nucleic acid sequence information was available. In this paper we describe a model methodology for PCR-mediated rescue of transfected genes from eukaryotic cell lines after functional selection. This protocol should allow any readily transfectable cell line to serve as a host for functional selection of cDNA expression libraries.
