Versaw W K
Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706.
Gene. 1995 Feb 3;153(1):135-9. doi: 10.1016/0378-1119(94)00814-9.
The pho-5+ gene of Neurospora crassa, which encodes a high-affinity phosphate permease, has been cloned and analyzed. The deduced ORF of 1707 nucleotides is interrupted by a single 63-nt intron and codes for a protein of 569 amino acids (aa). This aa sequence has 48% identity with the high-affinity phosphate transporter of Saccharomyces cerevisiae, PHO84. The pho-5 null mutants have no obvious phenotype. Strains which contain a null mutation in pho-4, which encodes an additional high-affinity phosphate permease [Bowman et al., J. Bacteriol. 153 (1983) 292-296], also have no obvious phenotype. However, strains containing mutations in both pho-5 and pho-4 are unable to grow under phosphate-restrictive conditions.
粗糙脉孢菌的pho-5+基因已被克隆和分析，该基因编码一种高亲和力的磷酸盐通透酶。推导的1707个核苷酸的开放阅读框被一个63个核苷酸的单一内含子中断，编码一个569个氨基酸（aa）的蛋白质。该氨基酸序列与酿酒酵母的高亲和力磷酸盐转运蛋白PHO84有48%的同一性。pho-5缺失突变体没有明显的表型。在编码另一种高亲和力磷酸盐通透酶的pho-4中含有缺失突变的菌株[Bowman等人，《细菌学杂志》153（1983）292 - 296]也没有明显的表型。然而，在pho-5和pho-4中都含有突变的菌株在磷酸盐限制条件下无法生长。
