粗糙脉孢菌中编码可阻遏碱性磷酸酶的pho-2+基因的克隆与特性分析
Cloning and characterization of the pho-2+ gene encoding a repressible alkaline phosphatase in Neurospora crassa.
作者信息
Grotelueschen J, Peleg Y, Glass N L, Metzenberg R L
机构信息
Biomolecular Chemistry Department, University of Wisconsin, Madison 53706.
出版信息
Gene. 1994 Jun 24;144(1):147-8. doi: 10.1016/0378-1119(94)90223-2.
PMID:8026754
Abstract
The Neurospora crassa phosphate-repressible alkaline phosphatase-encoding gene pho-2+ was cloned and its nucleotide sequence was determined. An open reading frame was found that contains four introns and encodes a putative protein of 555 amino acids. 'Activator-independent expression' of ectopically integrated pho-2+ was observed, as noted before for ectopically integrated pho-4+.
摘要
克隆了粗糙脉孢菌磷酸可阻遏碱性磷酸酶编码基因pho-2+，并测定了其核苷酸序列。发现一个开放阅读框，它含有四个内含子，编码一个推定的含555个氨基酸的蛋白质。如之前对异位整合的pho-4+所观察到的那样，观察到异位整合的pho-2+的“非激活因子依赖性表达”。
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