丙酮丁醇梭菌ATCC 824中编码σ因子的基因序列及排列

Sequence and arrangement of genes encoding sigma factors in Clostridium acetobutylicum ATCC 824.

作者信息

Wong J, Sass C, Bennett G N

机构信息

Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251.

出版信息

Gene. 1995 Feb 3;153(1):89-92. doi: 10.1016/0378-1119(94)00818-d.

DOI:10.1016/0378-1119(94)00818-d

PMID:7883192

Abstract

The nucleotide sequence of a 2.7-kb region of Clostridium acetobutylicum ATCC 824 DNA containing three open reading frames was determined. They encoded homologs of three proteins of Bacillus subtilis, and the gene arrangement in both organisms was identical. The first gene, orfA, was 801-bp long; the 31-kDa (266 aa) product it encoded exhibited homology with the putative sigma E-processing enzyme. The second gene, sigE, was 708-bp long encoding a 27-kDa (235 aa) product; the third gene, sigG, was 774-bp long encoding a 30-kDa (257 aa) product. These two proteins showed high homology with sigma E and sigma G, two sporulation-specific sigma factors.

摘要

测定了丙酮丁醇梭菌ATCC 824 DNA中一个包含三个开放阅读框的2.7 kb区域的核苷酸序列。它们编码枯草芽孢杆菌三种蛋白质的同源物，并且两种生物体中的基因排列是相同的。第一个基因orfA长801 bp；它编码的31 kDa（266个氨基酸）产物与假定的σE加工酶具有同源性。第二个基因sigE长708 bp，编码一个27 kDa（235个氨基酸）的产物；第三个基因sigG长774 bp，编码一个30 kDa（257个氨基酸）的产物。这两种蛋白质与两种孢子形成特异性σ因子σE和σG显示出高度同源性。