Santangelo J D, Kuhn A, Treuner-Lange A, Dürre P
Institut für Mikrobiologie, Georg-August-Universität, Göttingen, Germany.
FEMS Microbiol Lett. 1998 Apr 1;161(1):157-64. doi: 10.1111/j.1574-6968.1998.tb12943.x.
The gene for the vegetative sigma factor A of Clostridium acetobutylicum was constitutively transcribed during growth and formed an operon together with dnaE. Sporulation-specific sigma factors E, G, and K were sequentially induced shortly before mature endospores could be detected. Maximal transcription in the course of spore formation was found to be in the order sigE-sigG-sigK, thus matching the pattern described for Bacillus subtilis. From primer extension experiments promoter structures could be deduced with high homology to the Bacillus consensus. Upstream of the spoIIGA-sigE operon a gene with significant similarity to ftsZ could be detected.
丙酮丁醇梭菌营养型σ因子A的基因在生长过程中持续转录，并与dnaE一起形成一个操纵子。在能够检测到成熟内生孢子前不久，芽孢形成特异性σ因子E、G和K被依次诱导。发现在孢子形成过程中的最大转录顺序为sigE-sigG-sigK，因此与枯草芽孢杆菌中描述的模式相符。通过引物延伸实验，可以推导出与枯草芽孢杆菌共有序列具有高度同源性的启动子结构。在spoIIGA-sigE操纵子上游可以检测到一个与ftsZ具有显著相似性的基因。
